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WT and TDRD3 KO HuH-7 cells transfected with plasmids expressing GFP-TDRD3, GFP-TOP3B or a control plasmid (GFP) were infected with DENV-2. (E) TOP3B overexpression rescues DENV-2 infection in TDRD3 KO cells. (D) Comparison of viral titers in the supernatants of WT HuH-7, TDRD3 KO and TOP3B KO cells infected with DENV-2. (C) TOP3B and TDRD3 levels assessed by western blotting with TOP3B and TDRD3 specific antibodies respectively from lysates of WT, TOP3B KO and TDRD3 KO HuH-7 cells. (A) TDRD3 levels assessed by western blotting with TDRD3 specific antibody from lysates of WT and TDRD3 KO HuH-7 cells (B) TDRD3 KO inhibits DENV-2 propagation percentage of DENV-2 infected HuH-7 cells was determined by immunofluorescence microscopy using an antibody to the envelope protein (left) comparison of viral titers in the media of WT and TDRD3 KO cell cultures infected with DENV-2 (right).
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Electronic address: is required for efficient replication of DENV-2. 8 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore.7 Department of Epigenetics and Molecular Carcinogenesis, MD Anderson Cancer Center, Smithville, TX, USA.6 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.5 Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA Institute of Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.4 Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA.3 Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.2 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA Transplant Division, Department of Surgery, University of Texas Medical Branch, Galveston, TX, USA.1 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.